Don't let him go. 别让他走了。 (Let him go. 祈使句)

Don't worry! He will take care of himself.

不要担心他会照顾自己的。

Don't be afraid! 不要害怕。

Don't be silly. Don't be such a fool! 别傻了。

Don't you lie to me again.! 不可以再骗我了。

Don't you ever do it again! 不可以再做那件事了。

Isn't he an American? 他不是美国人吗？ (Yes/No,.…)

Don't you like him? 你不喜欢他吗？

Aren't you supposed to be studying now? 你现在不是应该在读书吗？

Won't you come in ? 请进来。

Isn't it a lovely day? 天气真好。